New NSF MONET Publication in Bioconjugate Chem

A team from the Steinmetz lab performed bioconjugation of mal-PEG11-mal or mPEG12-mal with the physalis mottle virus-like particle. Through a series of chemical assays and characterization studies, they demonstrate that while monovalent maleimides are highly selective for cysteine modifications, bivalent maleimides exhibit cross-reactivity toward both lysine and cysteine residues. Overall, this study highlights how linker valency can influence the chemoselectivity of maleimide-based conjugation chemistry.

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